Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated. F or each of the Kool-Aid flavors, 2.0 g was weighed out from the packet and 5mL of water was mixed in with them each. mL of 0.1% NaCl solution was added to 100mL of bottled water. The six chromatography strips …show more content…
For the orange, the distance the band traveled was 39mm and the distance solvent traveled was 39mm and the Rf was calculated at 1mm. For strawberry the distance band traveled 38mm and the distance the solvent traveled was 41mm and the calculated Rf was .927mm. The colors for strawberry, orange and grape Kool-Aid are made with food dye. There is also salt that is found in Kool-Aid that is why when placed in the tubes containing %NaCl, the solvent caused the drops on the strips to travel so far. The results in this were expected. In this lab, human error could have possibly been that the salt wasn’t fully dissolved or even the Kool-Aid wasn’t fully dissolved. To fix this next time, both mixtures can be stirred a little longer. A third human error could have been when putting 20 drops into the test tubes, some drops were bigger than others causing there to be more than mL of mixture in the test tube. At the end of the lab, a red and yellow M&M were used to do a home material test. I dissolved the color off the shell with warm water and placed a dot of each color onto a strip of chromatograph and placed them in two individual
For this lab, zeolite and magnetized zeolite were synthesized and compared with charcoal to find out with would be the most effective in the sequestering of Procion Red dye. Finding the concentration and absorbance of each zeolite, magnetized zeolite, and charcoal, along with a calibration curve, the best adsorbent is determined. Charcoal was the overall best sequestration of the Procion Red dye, since the adsorbent was highest compared to the others. Introduction Pollution has increased in the environment over the years, so the purpose of this experiment is to find the best adsorbent of chemicals to reduce the pollution.
Question 4: List the 3 errors; • Adding too many drops of NaOH at the same time would affect the results because we can’t determine the exact equivalent point when the color changed. The results won’t be accurate and that will affect all the data that are dependent on the amount of NaOH to titrate. • Other error could be the hardness to notice a color change; we always use a white paper under the flask to determine when the color changes right away. And if we don’t use the white paper it will be hard to determine the color change and the amount of NaOH that was used to titrate it. • Also other source of error could be by not rising the burette with NaOH before we fill up with it, or it maybe they were rinsing it with a lot of NaOH which could affect the data recording for NaOH amount of titration.
The ingredients of food dyes are unsafe, unnatural and can make people very sick if not tested or handled correctly. Coal-tar and petroleum based dyes
Shifa Sayeed can you check if this is all in past tense and if it has personal pronouns? The purpose of this experiment was to observe and thoroughly analyze how different substances of dissimilar intermolecular forces acted in different scenarios of evaporation, evaporative cooling, and boiling. In the lab, the three substances tested and compared were Acetone, Acetic Acid, and Propanol.
A lot of lesson in this experiment, one is that people like different flavor of gum for different reasons, and two not all Gums are the same. Our possible source of error is that we could
Materials: 100 mL plastic beaker blue crayola marker magenta crayola marker whatman filter paper 10mL tap water plastic pitcher of water blue scissors brown school paper towels SAFE-T plastic view-thru ruler 0.5 mechanical pencil clock Using Paper Chromatography to Separate Ink-Lab sheet Method: Using a small plastic pitcher filled with room temperature water, pour 10mL of the water into a small plastic beaker. If the walls of the beaker are wet be sure to dry them with a paper towel. With your scissors cut the Whatman filter paper into two strips, then cut one end of each strip into a point. Measure approximately two centimeters above the tip of the point on one of the pieces of filter paper with a ruler and mark a straight horizontal
If there is a color change, then it is known that protein is present in the solution. Finally, lipids are tested. 5 mL of water are added to 5 mL of oil. 5 drops of Sudan 3 are added, and if the color changes, then lipids are present. Next, the McMush is tested.
We identified unknown A as acetaminophen. This is because unknown A had all the same tested properties as acetaminophen when we performed our tests. For unknown B we found it to be acetaminophen and caffeine. This is because like unknown A, unknown B properties were the same as acetaminophen, but unknown B was acetone soluble. Unknown B has similar properties to caffeine because it was acetone soluble and because caffeine was the only basic pH tested to change color during the FeCl3 test.
The material for this experiment will be: Control tasting paper (tasteless), 0.003 mg per PTC strip, 0.007 mg per PTC strip, water, small medicine cups for tasting, larger medicine cups for rinsing, tower papers and dark chocolate (72%). This experiment demands 24 students in the Genetics Lab class. Students are going to be asked the level of bitterness in 3 different PTC exponents: 0.003 mg per PTC strip, 0.007 mg per strip and dark chocolate (72%). Levels are from 0-5, (0 being least bitter-5 being extremely bitter).
Introduction The purpose of this lab is to use control variables to help identify different macromolecules. Biological systems are made up of these four major macromolecules: carbohydrates, lipids, proteins and nucleic acids. Carbohydrates are sugar molecules (monosaccharides, disaccharides, and polysaccharides) which make them the most abundant macromolecule on the earth. Lipids (oils and fats, phospholipids and steroids) are insoluble in water and perform many functions such as energy source, essential nutrients, hormones and insulators (Lehman, 1955).
The childhood snack that will be analyzed is Fruit Gushers. The following are the five ingredients in this snack: Red 40 (Allura Red), partially hydrogenated cottonseed oil, carrageenan, corn syrup, and monoglyceride. Red 40 is an azo dye that contains an N=N grouping which is called an azo group. This dye dissolves in water because it’s a salt and contains sulfonic acid.
Use these results to determine the product concentration, using Beer-Lambert’s Law: A= ɛCl (where A is the absorbance, ɛ is the molar absorptivity, C is the product concentration and l is the length of solution that the light passes through). Calculate the product concentrations at every minute for 10 minutes for all 7 of the test tubes using Beer-Lambert’s Law. Plot a graph of product concentration vs. time and then use the gradients of the 7 test tubes to determine the velocities of the reaction. After calculating the velocities, plot a Michaelis-Menten graph of velocity vs. substrate concentration.
A 50 mL buret was obtained and was washed with NaOH solution. After filling the buret with NaOH (titrant) and preparing the KHP (analyte) in the Erlenmeyer flask, the solutions were titrated. The volume used from the NaOH solution was recorded. C. Determination of the Acidity of Soft Drinks First, the soft drinks were heated.
Name : Terry-Ann Poorman Id # : 27120454 Lab# : 1 Lab Instructor: Mrs. Bryan Title : Aim : Discussion Questions: 1) What are the types of urine specimens? Random specimen This type of specimen is used for microscopic and chemical analysis. As the name suggest this sample can be a randomly collected specimen and at an unspecified times.
Conclusion The GC ethanol analysis method described above has a simple concept, its rapid, and extremely accurate, determining ethanol precisely without interference from other beverage components. With this method, it takes only 7 to 8 min to complete a sample analysis for the determination of ethanol content in a beverage sample. Analyst handling is minimized to prevent deviation in results or possible human error. This method requires a gas chromatograph and a digital integrator, both reasonably expensive and sophisticated pieces of equipment.