Methyl Erucate Analysis

5. Results and Discussions

5.1 Methyl erucate synthesis

5.1.1 Chemical analysis of erucic acid

Physicochemical properties of erucic acid used for the synthesis of sucrose erucate were analyzed. The results of analysis are depicted in Table 5.1

Table 5.1 Analysis of erucic acid

Acid value 166.03 ± 0.9 mg KOH/gm

Iodine value 78.04 ± 1.2 g I2/100gm

Saponification value 168.05 ± 0.8 mg KOH/gm

5.1.2 Analysis of methyl erucate

After synthesis and purification of synthesized methyl erucate general characteristics of product were tested. The results obtained are shown in Table 5.2 and Figure 5.1 Figure 5.1 Color of methyl erucate

Table 5.2 General characteristics of methyl erucate

Color Light yellow

Odour

Consistency

The results of chemical analysis

Figure 5.2 TLC of methyl erucate [Solvent: chloroform: hexane (1:1)]

Rf value = (Distance travelled by solute )/(Distance travelled by solvent front)

=

5.1.5 Spectroscopy analysis using Fourier Transform Infrared Spectroscopy (FTIR)

The infrared spectra of the purified methyl erucate showed the characteristics band at 1739 cm-1 which attributed to carbonyl ester band (Figure 5.3). The other absorptions attributed to the molecule are shown in Table 5.4. Also the IR absorption peak can be compared as shown in Figure 5.3 and Figure 5.4.

Table 5.4 Spectroscopic identification of erucic acid functional groups in FTIR

Absorption frequency range(cm-1) Absorption peak (cm-1) Type of vibration

3020-3100 2920 =C-H stretch of alkene

2850-3000 2852 C-H stretch of alkane

1705-1720 1710 C=O stretch of acid

1350-1480 1452 -CH bend of alkane

1210-1320 1284 C-O stretch of acid

675-1000 931 =C-H bend of alkene

720-725 721 C-H rock of alkane

Figure 5.3 FTIR spectra of erucic