Peroxidase Chemical Reaction Lab
Title: Enzymes
Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms. (Enzymes par. 1) They are very sensitive to their surroundings and highly reactive to the pH levels and temperature once exposed to either one. Temperature causes damage to the enzyme,
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While conducting this experiment readings were took every 15 seconds for 5 minutes which equals to a total of 20 readings. This experiment had four different trials, a base line trail, a pH 4 trail, a pH of 7 trail, and a pH of 8 trail. Then the results were recorded. The enzyme peroxidase was taken from the inside of a turnip (2g), then blended with 150ml of deionized water, and then poured through a coffee filter into a beaker for a smooth solution. Secondly the colorimeter had to be calibrated by placing a clean cuvette only containing the enzyme solution. In a clean test tube 8.9ml of deionized water, 0.1 guaiacol, and 0.1ml enzyme solution. There was not supposed to be anything that happened after doing this so move to the next step. Next was adding 0.3ml of hydrogen peroxide, then cover it with dura-film to invert the solution. Then fill the cuvette 2/3 full and put it in the colorimeter. So at this point the stopwatch should be ready to start time then record results every 15 seconds. Then continue and follow each step for all four
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