Chromatographic Decontamination: A Case Study
1 Chromatographic decontamination 1) ion trade Decontamination of steed Ig by particle trade strategies has been portrayed. These creators took after the technique for Ter Avest et.al. (1992) with minor adjustments, utilizing DE-52 cellulose or DEAE CL-6b. 1gram DE-52 cellulose in 6ml 0.01m phosphate cradle (PB) ph6.0 was included every ml of serum. The DEAE CL-6b gel was washed twice with 0.5m Hcl, twice with 0.5m Naoh and twice with PB ph6.0 before utilization. For DEAE 1ml gel every 1ml serum was utilized. In the wake of blending for 1 hour at 200c the suspension was centrifuged at 4500g for 25 minutes. The supernatant was thought by Ammonium sulfate precipitation and pellet was broken up in refined water and the ensuing arrangement was dialyzed against PBS ph7.2 to evacuate Ammonium sulfate and conformed to the first volume with yhe same cushion (Hong et.al., 1994). 2) …show more content…
Quickly the serum was acclimated to 0.75m Sodium sulfate and 3 volumes of Affi-T gel was included under tender shaking at 200c. following 1 hour the gel was washed three times with three volumes of 0.75m Ammonium sulfate precipitation and the ensuing arrangement was conformed to the first volume as depicted above (Honge et.al., 1994). 2.18.2 Non-Chromatographic
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680 Words | 3 PagesUsing two separate aseptic pipettes, 250 µl of LB broth were added to each micro test tube and mixed gently. Likewise, using two separate, aseptic pipettes for each tube, 100 µl of solution was added to the appropriate agar plate. After, using a new loop for each plate, the solution was spread gently across their surfaces. Lastly, the plates were stacked, taped together, and labelled before placing them upside down in an incubator set at 37°C
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313 Words | 2 Pages- The calculated TWA for the personal sample is (0.041 ppm), which did not exceed OSHA-PELs (0.75 ppm) for Formaldehyde, but did exceed both ACGIH-TLVs (0.3 ppm) and NIOSH-RELs (0.016 ppm) for Formaldehyde. - The calculated TWA for the desk area sample is (0.022 ppm), which did not exceed OSHA-PELs (0.75 ppm) for Formaldehyde nor did it exceed ACGIH-TLVs (0.3 ppm), but it did exceed the NIOSH-RELs (0.016 ppm) for Formaldehyde. - The calculated TWA for the cadaver area sample is (0.032 ppm), which did not exceed OSHA-PELs (0.75 ppm) for Formaldehyde, but did exceed both ACGIH-TLVs (0.3 ppm) and NIOSH-RELs (0.016 ppm) for Formaldehyde. • Discussion and Conclusion: To calculate the TWA a similar exposure method was used to assume worst case scenario. The results showed that the worker was not exposed to Formaldehyde concentrations exceeding OSHA-PELs in all calculated TWAs, which are the maximum exposure limits allowed under Federal law, which should be followed by the employers to protect the health of their workers.
Hand Sanitizer Case Study
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Presence Of Macromolecules In Mcdonald's Happy Meals
1184 Words | 5 PagesStarch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
Serum 2216 Lab Report
592 Words | 3 PagesThe investigation was carried out to identify the presence or absence of biological molecules in serum 2216. If the concentration in each test tube of the dilutions carried out will be more concentrated then the concentration of the test tube before it, then the color will be at an equal concentration with the other dilutions performed. The hypothesis was wrong because of the difference in concentrations due to the different measurements within the dilutions done. The test for starch was to add a drop of iodine solution to the pipette in the spotting tile. A reducing sugar solutions is add inside a test tube with 3 drops to then add 3 drops of benedicts and plane in a water bath.
Dot Blot Analysis
813 Words | 4 PagesThe Dot blot methodology offers significant savings in time, the technique can be done less than an hour as the procedures for the gel such as gel electrophoresis and chromatography are not required. However, due to unavailable information on the size of target molecules. The dot blot
Cupric Sulfate Hypothesis
473 Words | 2 PagesThe Correlation Between Paramecium and Cupric Sulfate Hypothesis: The class came to the decision to use cupric sulfate as the collaborative experiment we were about to perform. Prior, each group performed similar experiments, where each group added different chemicals to paramecium cells to see if the chemical will have any effect on their speed. After each group presented their findings, we decided to remake the experiment using the chemical group 2 used, cupric sulfate. The class came to the alternative hypothesis that the presence of cupric sulfate will affect the speed of the paramecium cells.
Rlp Analysis Of Dna Fingerprinting
1055 Words | 5 PagesFor this reason the DNA is denatured into single strands by incubation with sodium hydroxide solution (NaOH). If the DNA fragments are larger than 15kb, the gel can be treated with acid such as dilute HCl prior to blotting. The DNA is depurinated by the acid and breaks down into smaller pieces which allows more efficient transfer from the gel to the membrane. The Southern Blot Method begins with the DNA fragments being transferred to a nitrocellulose membrane.
Erythrocyte Membranes Lab Report
865 Words | 4 PagesAnything that has a band in another of the samples which is also found in the membrane sample is contamination and has not been identified. The 15% Gel also has another contaminant below the band identified as stomatin, as this protein is found in every sample from plasma to membrane. The 7% gel only contains one obvious contaminant which is the same as that found on the 15% gel as its below
Essay On Ion Exchange Chromatography
966 Words | 4 PagesIon Exchange Chromatography is a technique for ionic separation based on exchange with resins in stationary phase and the eluents in mobile phase. These stages are based on the exchanges in an anion column to attract anions or in a cation column to attract cations. cations. A column measures the conductivity of a particular ion based on its affinity/attraction to it. The speed of movement of ions through the ion chromatograph columns depends not only on the diameter of the column but basically on the affinity of the ion to the specific resin or elute selected, the size of the interacting molecules and also the resultant distance between them based on the degree of attraction and repulsion.
Orthophosphoric Acid Solution
815 Words | 4 PagesA mobile phase system consisting of acetonitrile and 25mM phosphate buffer of pH 3(sodium dihydrogen phosphate monohydrate adjusted with orthophosphoric acid) in a ratio 60:40 (v/v) were used. The mobile phase was degassed and filtered by passing through 0.45 µm pore size membrane filter (Millipore, Milford, MA, USA) prior to use. The flow rate was 1.0 mL min-1 all over the run. The injection volume was 20 µL. The eluent was monitored by the diode array detector (DAD) which was set at 250 nm for the quantitation of both VAL and SAC.
Platelet Activating Factor Case Study
1010 Words | 5 PagesThe test used was Enzyme-linked immunosorbent assay (ELISA) which is a test that uses antibodies to see a change in color to identify an antigen. Fresh venous blood samples were drawn into pyogen-free blood collection tubes without additives, immersed in ice, and allowed to clot before centrifugation. All serum samples were stored at -70°C. Serum PAF-AH levels were measured with ELISA kits. The detection limit of this assay was 0.074 ng/ml.2
Fatty Acid Methyl Ester Mix (DPPH)
1359 Words | 6 PagesMaterials and methods Standards and reagents Fatty acid methyl ester mix (PUFA No 3 from menhaden oil) was purchased from Supelco (USA). Folin–Ciocalteu reagent, gallic-acid, phloroglucinol, 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), ascorbic acid and BHT were purchased from Sigma-aldrich, (Germany); sodium carbonate, YPD medium and Mueller-Hinton agar were purchased from Merck (Germany); Chloramphenicol, Ciprofloxacin and McFarland scale were purchase from Biomerieux (France). Methanol, n-Hexane and dichloromethane were purchased from Fisher Scientific (U. K). Dimethyl sulfoxide, 3-[4, 5-dimethylthiazol-2-yl]-2 5-diphenyl tetrazolium bromide (MTT) and cisplatin were purchased from Sigma-Aldrich (U.S.A.); isopropanol was purchased from Panreac
The Importance Of Cleaning In Health Care
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