Thin Layer Chromatography Lab Report
The molecular formula is a way to tell if a structure is polar or not. Thin layer chromatography was used to see the polarity of components in a mixture. The silica gel on the TLC plate and the mixture can tell the relative Rf or Rf of the different spots on the plate. On the TLC plate, the order was β-carotene, pheophytin a or b, chlorophyll a, chlorophyll b, and then lutein. The relative Rf for β-carotene was closer to one compared to the other pigments, so β-carotene was more polar then the rest of the pigments. On the hand, pheophytin a or b, chlorophyll a, chlorophyll b, and lutein were less polar. The observations of the four pigments in UV light showed which pigment had red in them. The β-carotene had no red in the sample. The chlorophyll
Paper Chromatography Lab
647 Words | 3 PagesIn science class, we did an experiment to compare inks and their compositions. This was intended for us to learn about chromatography; the separation of a mixture through a medium (the chromatography paper) in which the components move at different rates and create different patterns. We chose to explore this topic by using four different colored markers, placing a dot of each color onto one piece of chromatography paper, and observing while the water in the beaker travels up the paper and separates the dyes. Before we started, we made a hypothesis. Mine was if paper chromatography is done on the ink in the green, red, brown, and black marker, then the green will spread out into different shades of blue and green making a pointed oval shape.
Cell Membrane Permeability Case Study
1977 Words | 8 PagesThe Beetroot contains a red pigment known as betanin. This pigment was first discovered by schudel in the year 1918. They are classified under one of the two categories of betalains; Betacyanins and They are the most common in the plant kingdom. Betanin has also been classified as an anthocyanin related to the pigments of the petals of many flowering plants but different from typical anthocyanins because it contains nitrogen (Pucher, et al., 1937). The absorbance of this red pigment; betanin was tested in this lab in relation to the membrane permeability of the beet plant.
Substrate Concentration Lab Report
181 Words | 1 PagesHypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.
Spinach Leaf Photosynthesis Lab Report
1169 Words | 5 PagesHypothesis: If four lights (UV Light, LED Light, Infrared Light, and Blue Light) are used to perform photosynthesis on a spinach leaf, then the spinach will respond most to the blue light and perform a more efficient photosynthetic process.
Temporary Emulsions Lab Report
449 Words | 2 PagesFats, Soups, Sauces and Emulsions In my lab today I got to make some soup, biscuits, sauce, vanilla pudding, mayonnaise as well as oil and vinegar salad dressing. Emulsion is a process where a liquid is dispersed in another liquid with which it is usually immiscible. There are two types of emulsions. Oil-in-water (oil droplets are dispersed throughout the water) and water-in-oil (water droplets are dispersed throughout the oil).
Photosynthesis: The Chemical Equation Of Photosynthesis
1896 Words | 8 Pages(From Light Energy to Chemical Energy, Accessed 08 March 2018). Products to are made through photosynthesis as glucose is transported in to the plant as soluble sugars and stored as starch which is insoluble hence it is unable to escape from the cell. Although starch is able to revert back into glucose in order to release energy by respiration both useful for fats and oils for storage through the plant, proteins for growth and repair and cellulose for their cell walls. Figure 2: Structure of a leaf (FTEXPLORING, 2014), Accessed 16 March 2018) Chlorophyll is an essential need to photosynthesis as it is able to trap both blue and red light. It is a complex molecule consisting of three sub types;
Clinical Macula Case Study
883 Words | 4 PagesThe pigments differ slightly in their chemical properties and subsequently in their relative ability to absorb light of different wavelengths. These cones are loosely called "blue", "green", and "red" as they are supposed reflect their peak sensitivities on
Lycopene Lab Report
400 Words | 2 PagesIntroduction Carotenoids are pigments that are found mainly in chloroplasts of plants and in some photosynthetic organisms. They are synthesised naturally in plants in order to absorb light during photosynthesis and protect plants against photosensitization. They are found in our diet, through eating fruit and vegetables and many serve as an important source of vitamin A in our diets, such as ß-carotene. The structure of the molecule determines the colour, photochemical properties, vitamin activity and its chemical reactivity on oxidising agents and free radicals.
Essay On The Effect Of Light On Photosynthesis
2543 Words | 11 PagesHowever, I did not know that sunlight and water are essential for plants’ growing. So, the plants just get rotten. Therefore, through this experiment, I am able to find out the effects of colors in photosynthesis. Absorption spectrum is related to my experiment which shows that green color is the poorly absorbed and red and blue color would be absorbed well. Moreover, plants are necessary for us since we inhale oxygen which is produced by plants.
Effect Of Enzyme Concentration On Enzyme Reaction
1046 Words | 5 PagesThe 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
Amino Acids Lab Report
1410 Words | 6 PagesThe chromatography solvent may have created a few issues as well. In a first attempt at the experiment, none of the leaf pigments separated from the origin. The solvent that was used was from a bottle of reused chromatography solvent. Once the solvent was used straight from its original bottle, the pigments began to separate. In addition, the solvent was not always at equal levels as it was difficult to precisely measure with the pipet.
Column Chromatography Lab Report
1696 Words | 7 PagesExperiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.
Column Chromatography Essay
1137 Words | 5 Pagesand NMR spectra were recorded in CD3OD and CDCL3 using a Bruker WM 500 spectrometer at room temperature [500 MHz (1 H NMR) and 125 MHz (13C NMR)]. From the results of spectral analysis found;9-hexanoyl-3-(2-hydroxypropyl)-6a-methyl-9,9a-dihydro-6H-furo[2,3-h]isochromene-6,8(6aH)-dioneand4-[2,4-dihydroxy-6-(3-hydroxybutanethioyloxy)-3methylphenyl]-3,4-dihydroxy-3,6-dimethylheptanoic acid. These pigments may have been better to used as colorants in food industry (Campoy S et al.
Beta Carotene Research Paper
804 Words | 4 Pages3. Identification of Beta carotenes. Focus of this review is the identification of beta carotenes in the infrared spectrum region. In terms of metabolism and potential effects on health, beta carotene is one of the examples of most studied carotenoids. Thus the basic structure of beta carotene is made up of isoprene units.
Essay On Cuttlefish
1011 Words | 5 PagesThis ability make the animals blend with their surroundings not only for camouflage but also for communication such as during mating. The pigment cells (chromatophores) are responsible for releasing the pigment into the outer layer of animal’s skin. The pigmented chromatophores with its variety of colors (e.g. yellow, red, orange, brown, black, blue and green) can -when used in combinations- are responsible for the numerous colors which cuttlefish can display. It is claimed that cuttlefish are the fastest color changers in the whole animal kingdom.
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